Biopsy


Staining method


Pathology


Hematoxylin–eosin (HE)


Evaluation of muscle fiber size and shape, vascular and nerve structures, infiltrates, general histology (Fig. 9.1)


Gomori trichrome


Detection of ragged red fibers (mitochondrial dysfunction, Fig. 9.2), rimmed vacuoles (inclusion bodies, Fig. 9.3)


Oil red O


Evaluation of lipid deposition (Fig. 9.4)


Periodic acid–Schiff (PAS)


Glycogen deposition


Acid phosphatase


Lysosomal enzyme, indicative of degenerative changes of muscle fibers


Cytochrome C oxidase (COX)


Mitochondrial enzyme (Fig. 9.6)


Succinate dehydrogenase (SDH)


Mitochondrial enzyme


ATPase


Differentiation between type I and type II muscle fibers;.ber type grouping (Fig. 9.7)


NADH-tetrazolium reductase (NADH-TR)


Enzyme in mitochondria, T tubules and sarcoplasmic reticulum, target fibers


image


Fig. 9.1 Histology of normal muscle (HE stain). The section shows muscle fibers of normal size and shape, and normal vascular and nerve structures. Infiltrates are absent.


image


Fig. 9.2

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Jun 4, 2016 | Posted by in NEUROLOGY | Comments Off on Biopsy

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