Induced Experimental Autoimmune Encephalomyelitis in Rats


Straina

Haplotype

Class I

Class II

Class III

Class I

RT1.A

RT1.B

RT1.D

RT1.C

DA

av1

a

a

a

av1

av1

COP

av1

a

a

a

av1

av1

ACI

av1

a

a

a

av1

av1

PVG-RT1AV1 (DA)

av1

a

a

a

av1

av1

LEW.1AV1 (DA)

av1

a

a

a

av1

av1

LEW

l

l

l

l

l

l

LEW.1N (BN)

n

n

n

n

n

n

LEW.1A (AVN)

a

a

a

a

a

a

LEW.1W (WP)

u

u

u

u

u

u

LEW.1AR1

r2

a

u

u

u

u

LEW.1AR2

r3

a

a

a

u

u

LEW.1WR1

r4

u

u

u

a

a

LEW.1WR2

r6

u

a

a

a

a

BN

n

n

n

n

n

n


RT1.B is the rat equivalent to HLA-DQ and RT1.D to HLA-DR

aDonor strain in brackets




Table 2
Presently most used EAE models for laboratory research




























Species
Strain
Induction
Disease type
Reference

Rat
LEW
MBP or MBP 68–88 peptide in CFA
Acute monophasic
(11, 12)

Rat
DA
Spinal cord in IFA or MOG 1–125 in CFA or IFA
Relapsing/progressive
(5, 13)


The list only provides a part of all available EAE models

CFA complete Freund’s adjuvant, IFA incomplete Freund’s adjuvant



Table 3
Immunization regimen for different EAE models and usage of adjuvants



































































Strain
Immunogen
Primary adjuvant
Secondary adjuvant
Injection site
Reference

LEW
MBP
CFA
None
Tail base
(14)

LEW
MBP 68–88
CFA
None
Tail base
(5)

LEW
MBP 89–101
CFA
None
Tail base
(14)

LEW
PLP
CFA
None
Tail base
(15)

DA, LEW.1AV1, BN, LEW.1N, LEW1.AR1
MOG 1–125
CFA or IFA
None
Tail base
(5, 8, 16)

DA, LEW.1AV1
MOG 91–108, MOG 96–104
CFA
None
Tail base
(9, 10)

LEW.1N or BN
MOG 91–108, MOG 98–106
CFA
None
Tail base
(9, 10)


CFA complete Freund’s adjuvant, IFA incomplete Freund’s adjuvant



Table 4
EAE models induced with MOG 1–125 in rats






































































Species
Strain
Induction
Disease type
Reference

Rat
LEW.1A
MOG 1–125 in CFA
Chronic progressive
(5)

Rat
LEW.1AV1
MOG 1–125 in CFA
Relapsing-remitting
(5)

Rat
LEW.1AV1
MOG 1–125 in IFA
Relapsing-remitting
(16)

Rat
LEW.1N
MOG 1–125 in CFA
Hyperacute progressive
(5)

Rat
LEW.1N
MOG 1–125 in IFA
Chronic progressive
(16)

Rat
LEW.1W
MOG 1–125 in CFA
Slow progressive
(5)

Rat
LEW.1AR1
MOG 1–125 in CFA
Slow progressive, cortical pathology
(5, 8)

Rat
DA
MOG 1–125 in CFA
Relapsing-remitting, optic neuritis
(5, 6)

Rat
BN
MOG 1–125 in CFA
Neuromyelitis optica
(5, 17)


Different disease courses and types of CNS pathology can be induced that are dependent on the MHC haplotype



Table 5
EAE models induced with MOG peptides
























Species
Strain
Induction
Disease type
Reference

Rat
DA
MOG 91–108 in CFA
Monophasic or chronic
(9)

Rat

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Jul 12, 2017 | Posted by in NEUROLOGY | Comments Off on Induced Experimental Autoimmune Encephalomyelitis in Rats

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