Induced Experimental Autoimmune Encephalomyelitis in Rats


Straina

Haplotype

Class I

Class II

Class III

Class I

RT1.A

RT1.B

RT1.D

RT1.C

DA

av1

a

a

a

av1

av1

COP

av1

a

a

a

av1

av1

ACI

av1

a

a

a

av1

av1

PVG-RT1AV1 (DA)

av1

a

a

a

av1

av1

LEW.1AV1 (DA)

av1

a

a

a

av1

av1

LEW

l

l

l

l

l

l

LEW.1N (BN)

n

n

n

n

n

n

LEW.1A (AVN)

a

a

a

a

a

a

LEW.1W (WP)

u

u

u

u

u

u

LEW.1AR1

r2

a

u

u

u

u

LEW.1AR2

r3

a

a

a

u

u

LEW.1WR1

r4

u

u

u

a

a

LEW.1WR2

r6

u

a

a

a

a

BN

n

n

n

n

n

n


RT1.B is the rat equivalent to HLA-DQ and RT1.D to HLA-DR

aDonor strain in brackets




Table 2
Presently most used EAE models for laboratory research




























Species

Strain

Induction

Disease type

Reference

Rat

LEW

MBP or MBP 68–88 peptide in CFA

Acute monophasic

(11, 12)

Rat

DA

Spinal cord in IFA or MOG 1–125 in CFA or IFA

Relapsing/progressive

(5, 13)


The list only provides a part of all available EAE models

CFA complete Freund’s adjuvant, IFA incomplete Freund’s adjuvant



Table 3
Immunization regimen for different EAE models and usage of adjuvants



































































Strain

Immunogen

Primary adjuvant

Secondary adjuvant

Injection site

Reference

LEW

MBP

CFA

None

Tail base

(14)

LEW

MBP 68–88

CFA

None

Tail base

(5)

LEW

MBP 89–101

CFA

None

Tail base

(14)

LEW

PLP

CFA

None

Tail base

(15)

DA, LEW.1AV1, BN, LEW.1N, LEW1.AR1

MOG 1–125

CFA or IFA

None

Tail base

(5, 8, 16)

DA, LEW.1AV1

MOG 91–108, MOG 96–104

CFA

None

Tail base

(9, 10)

LEW.1N or BN

MOG 91–108, MOG 98–106

CFA

None

Tail base

(9, 10)


CFA complete Freund’s adjuvant, IFA incomplete Freund’s adjuvant



Table 4
EAE models induced with MOG 1–125 in rats






































































Species

Strain

Induction

Disease type

Reference

Rat

LEW.1A

MOG 1–125 in CFA

Chronic progressive

(5)

Rat

LEW.1AV1

MOG 1–125 in CFA

Relapsing-remitting

(5)

Rat

LEW.1AV1

MOG 1–125 in IFA

Relapsing-remitting

(16)

Rat

LEW.1N

MOG 1–125 in CFA

Hyperacute progressive

(5)

Rat

LEW.1N

MOG 1–125 in IFA

Chronic progressive

(16)

Rat

LEW.1W

MOG 1–125 in CFA

Slow progressive

(5)

Rat

LEW.1AR1

MOG 1–125 in CFA

Slow progressive, cortical pathology

(5, 8)

Rat

DA

MOG 1–125 in CFA

Relapsing-remitting, optic neuritis

(5, 6)

Rat

BN

MOG 1–125 in CFA

Neuromyelitis optica

(5, 17)


Different disease courses and types of CNS pathology can be induced that are dependent on the MHC haplotype



Table 5
EAE models induced with MOG peptides
























Species

Strain

Induction

Disease type

Reference

Rat

DA

MOG 91–108 in CFA

Monophasic or chronic

(9)

Rat

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Jul 12, 2017 | Posted by in NEUROLOGY | Comments Off on Induced Experimental Autoimmune Encephalomyelitis in Rats
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