Osteopontin for Rodent Germinal Matrix Hemorrhage

Fig. 1

Developmental milestone, measured by negative geotropism (seconds), 72 h after collagenase infusion; (asterisk) <0.05 compared with sham; (cross) <0.05 compared with GMH (vehicle); SEM standard error of the mean; n = 6/group

Fig. 2

Hemoglobin assay, measured by spectrophotometer, 72 h after collagenase infusion; (asterisk) <0.05 compared with sham; SEM standard error of the mean; n = 4/group

Fig. 3

Evans blue, measured by spectrophotometer, 72 h after collagenase infusion; (asterisk) <0.05 compared with sham; (cross) <0.05 compared with GMH (vehicle); SEM standard error of the mean; n = 4/group

Conclusion

OPN may play an important role in enhancing neuroprotective brain signaling following GMH. Translational stroke studies, including animal modeling, are greatly needed to safely integrate basic preclinical scientific principles ahead of clinical application [24–28]. Intranasal OPN improved outcomes after GMH by attenuation of brain swelling, BBB function, re-bleeding, and neurological outcomes. These observed effects may offer novel possibilities for eventual therapy in this patient population. Further study is needed to evaluate the mechanisms of this neuroprotection.

Acknowledgment

This study was partially supported by the National Institutes of Health grant RO1 NS078755 (Dr. Zhang) and American Heart Association CRP 17380009 (Dr. Lekic).

Disclosures

None

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