and Mario Di Napoli1
(1)
Neurological Service, S. Camillo de’ Lellis General Hospital, Rieti, Italy
Original Settings
Sensitivity was 20 μV/div (digit–wrist) and 50 μV/div (palm–wrist), low-frequency filter was 2 Hz, high-frequency filter was 10 kHz, duration of pulse was 0.1 ms, and rate of pulse was approximately 1/s. Sweep speed and the machine used were not specified.
Position
This study was performed in the supine position.
Recording
Following the antidromic method [1], signals were recorded using surface ring electrodes placed distally to the digits from the proximal stimulation of the median nerve (S) on the palm and at the wrist. Sensory nerve action potentials (SNAPs) were recorded from digit I (thumb), digit II (index), digit III (middle), and digit IV (ring). Active ring (A) electrode was placed on the proximal interphalangeal joint of digit I, digit II, digit III, and digit IV; the reference ring (R) electrode was placed on the distal (R) interphalangeal joint of the same digits. Each wrist–digit recording was made separately, at a 14 cm distance between the recording and the stimulating electrodes which was used for each wrist–finger test (Figs. 1, 2, 3, and 4). To study the palm–digit segment, the authors performed stimulation on the palm recording SNAPs using ring electrodes from digit II and digit III (Figs. 5 and 6). Ground (G) electrode position was not mentioned in the text; the figures show the ground electrode positioned on the palm.







Fig. 1
Antidromic sensory nerve action potential (SNAP) recorded to digit I, stimulation at the wrist

Fig. 2
Antidromic sensory nerve action potential (SNAP) recorded to digit II, stimulation at the wrist

Fig. 3
Antidromic sensory nerve action potential (SNAP) recorded to digit III, stimulation at the wrist

Fig. 4
Antidromic sensory nerve action potential (SNAP) recorded to digit IV, stimulation at the wrist

Fig. 5
Antidromic sensory nerve action potential (SNAP) recorded to digit II, stimulation on the palm

Fig. 6
Antidromic sensory nerve action potential (SNAP) recorded to digit II, stimulation on the palm
Stimulation
Stimulations were applied to the median nerve proximal to the distal wrist crease and on the palm. For wrist stimulation (S), the median nerve was stimulated on a surface point between the tendons of the flexor carpi radialis and the palmaris longus muscles (ideally proximal to the distal wrist crease). Wrist stimulation was made at a fixed 14 cm distance from the active ring (A) recording electrode placed distally to the digit. For palm stimulation (S), the median nerve was stimulated at a fixed 8 cm distance for both palm–digit II and palm–digit III segments. The interelectrode distance between the cathode (−) and anode (+) of the stimulating electrodes was 3 cm. The authors did not specify if surface disk electrodes (the same used for wrist recordings) or a handheld bipolar stimulator was used for palm stimulation.
Measurements
Onset latency (ms) was measured from the onset of the stimulus to the onset of the negative deflection of the evoked sensory nerve action potential (SNAP); peak latency (ms) was measured from the onset of the stimulus to the negative peak of the wave. The negative peak amplitude (μV) was measured from the stimulus artifact to the peak of the negative deflection of the SNAP; peak to peak amplitude was measured from the negative to the positive deflection of the wave. Durations (ms) were recorded from onset of the negative deflection to its return to the baseline and from the onset of the negative wave to the positive peak. To establish the antidromic conduction in the palm–wrist segment, the latency value was taken from the latency measured from the wrist to finger. Temperatures were monitored at the point of stimulation and at the point of recording. All tests were performed with stable temperatures ranging from 31 to 33 °C (the majority were 32 °C), when necessary the limb was warmed with an infrared lamp. Normal values were obtained from 30 median nerves of 30 healthy subjects (age range 22–58 years, mean age 37.8 years). The side to side latency difference was analyzed from 15 subjects who were tested bilaterally.
Onset latency | Mean ± SD | Side to side |
---|---|---|
Wrist–digit I – 14 cm (ms) | 2.3 ± 0.2 | 0.3 |
Wrist–digit II – 14 cm (ms) | 2.3 ± 0.2 | 0.3 |
Wrist–digit III – 14 cm (ms) | 2.3 ± 0.2 | 0.5 |
Wrist–digit IV – 14 cm (ms) | 2.4 ± 0.2 | 0.5 |
Peak latency | Mean ± SD | Side to side |
---|---|---|
Wrist–digit I – 14 cm (ms) | 3.0 ± 0.2 | 0.4 |
Wrist–digit II – 14 cm (ms) | 2.9 ± 0.2 | 0.5 |
Wrist–digit III – 14 cm (ms) | 3.0 ± 0.2 | 0.6 |
Wrist–digit IV – 14 cm (ms) | 2.9 ± 0.2 | 0.5 |
Negative peak amplitude | Mean ± SD | Side to side |
---|---|---|
Wrist–digit I – 14 cm (μV) | 32.9 ± 10.5 | |
Wrist–digit II – 14 cm (μV) | 42.0 ± 10.2 | 4.4 |
Wrist–digit III – 14 cm (μV) | 44.3 ± 13.6 | 6.6 |
Wrist–digit IV – 14 cm (μV) | 29.1 ± 10.6 | 7.8 |
Peak to peak amplitude | Mean ± SD | Side to side |
---|---|---|
Wrist–digit I – 14 cm (μV) | 43.8 ± 15.3 | |
Wrist–digit II – 14 cm (μV) | 53.9 ± 17.3 | 9.8 |
Wrist–digit III – 14 cm (μV) | 61.9 ± 17.0 | 12.3 |
Wrist–digit IV – 14 cm (μV) | 38.2 ± 15.0 | 11.8 |
Baseline duration | Mean ± SD | Side to side |
---|---|---|
Wrist–digit II – 14 cm (ms) | 1.1 ± 0.2 | 0.2 |
Wrist–digit III – 14 cm (ms) | 1.0 ± 0.2 | 0.1 |
Wrist–digit IV – 14 cm (ms) | 1.0 ± 0.1 | 0.1 |
Positive peak duration | Mean ± SD | Side to side |
---|---|---|
Wrist–digit II – 14 cm (ms) | 1.4 ± 0.2 | 0.2 |
Wrist–digit III – 14 cm (ms) | 1.4 ± 0.2 | 0.2 |
Wrist–digit IV – 14 cm (ms) | 1.3 ± 0.2 | 0.2 |
Onset latency | Mean ± SD | Side to side |
---|---|---|
Palm–digit II – 6 cm (ms) | 1.2 ± 0.2 | 0.7 |
Palm–digit III – 6 cm (ms) | 1.3 ± 0.2 | 0.8 |
Peak latency | Mean ± SD | Side to side |
---|---|---|
Palm–digit II – 6 cm (ms) | 1.4 ± 0.2 | 0.8 |
Palm–digit III – 6 cm (ms) | 1.4 | 0.6 |
Comment
For the digit–wrist segment with a distance of 14 cm between the stimulating and recording electrodes, the mean nerve conduction latency found by Di Benedetto et al. [1] was 60.8 m/s (average latency of 2.3 ms). Maximal side to side difference observed was 0.3 ms for the measurements to the onset and 0.5 ms for the measurement to the peak. Mean latency for conduction over the 8-cm segment between the wrist and palm was 1.3 or 1.4 ms when measured to the onset and 1.8 or 1.9 ms when measured to the peak. Mean latency for conduction over the 6 cm segment between the palm and digit was 1.2 or 1.3 ms measured to the onset and 1.3–1.4 ms measured to the peak. Amplitudes of the orthodromic responses recorded at the wrist (14 cm) were only about 34 % of the amplitudes of responses conducted antidromically over the same nerve segment. The duration of evoked potentials were conducted over a 14-cm distance and recorded with active and reference electrodes spaced at 3 cm (the mean side to side difference was 0.15 ms, and the maximal difference was 0.5 ms). Measurements were most consistent when examining only the duration of the negative wave.
Lew et al. [2] studying 44 median nerves from 44 healthy (Table 3) subjects (44 right hands, 24 men and 20 women, mean age 44.0 ± 12.9 years, age range 25–80 years) performed the antidromic sensory techniques (long-distance method) to digit II, digit III, and digit IV using the method by Di Benedetto et al. [1]. For digit I antidromic recordings (Fig. 7), they stimulated the median nerve at the wrist – 10 cm distance (measured in a straight line with digit I extended) – using the method by Kothari et al. [3]. The active recording (A) electrode was separated from the reference (R) electrode by 3 cm. They used a Nicolet Viking IV electromyograph (filter settings 20 Hz–3 kHz, gain 20 μV/div, and a sweep speed of 1 ms/div). Onset latencies and peak latencies were used to calculate SNCV in the long segment.


Fig. 7
Antidromic study to digit I (10 cm fixed distance)
SNCV – onset latency | Mean ± SD | Limit of normal |
---|---|---|
Wrist–digit I – 10 cm (m/s) | 48.7 ± 5.0 | 38.7 |
Wrist–digit II – 14 cm (m/s) | 55.6 ± 4.4 | 46.8 |
Wrist–digit III – 14 cm (m/s) | 56.1 ± 4.3 | 47.4 |
Wrist–digit IV – 14 cm (m/s)
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