Fig. 1 Ventilation system/monitoring setup. A typical setup for intravital microscopy is indicated. Animals are supplied with medical oxygen and isoflurane via a volume-controlled animal ventilator. Physiological parameters are continuously…
MOG Forward 5′-AAA GAATTCGATGGCCTGTTTGTGGAGCT-3′ MOG Reverse 5′-AAA GCGGCCGCCAGGAAGACACAACCATCACTCA-3′ MBP Forward 5′-AAA GAATTCTAGCCTGGATGTGATGGCAT-3′ MBP Reverse 5′-AAA GCGGCCGCCAGGATTCGGGAAGGCTGA-3′ PLP Forward 5′-AAA GAATTCAAGTGCCAAAGACATGGGCT-3′ PLP Reverse 5′-AAA GCGGCCGCGCTCAGAACTTGGTGCCT-3′ All primers were designed with software…
Fig. 1 After density gradient centrifugation, different blood cells are found in different layers according to their density. From bottom to top you find red blood cells and granulocytes, followed…
Antibody Clone Provider* CD1d LY38 1 CD3 SP34-2 1 CD4 L200 1 CD5 UCHT2 3 CD8 LT8 4 CD9 ML13 1 CD11b D12 1 CD11c S-HCL-3 1 CD16 3G8 1…
Fig. 1 Illustration of full-length MOG fused to EGFP as displayed by the transfected cells. The cartoon of MOG is based on Fig. 1b from (18) Fig. 2 Quantitative analysis…
Fig. 1 Flow cytometric staining of the inflammatory infiltrate at the peak of EAE. (a) CD4 versus CD11b staining of CNS cells (initially gated using a live leukocyte gate). (b)…
Fig. 1 Immunohistochemistry, confocal fluorescence microscopy, and in situ hybridization techniques. (a, b, c, bar: 1 cm) Hemispheric sections from the brain of an RRMS patient. (a) Luxol Fast Blue/periodic acid…
Straina Haplotype Class I Class II Class III Class I RT1.A RT1.B RT1.D RT1.C DA av1 a a a av1 av1 COP av1 a a a av1 av1 ACI av1…
Fig. 1 Time schedule for oocyte production and retransfer First injection: 200 μl GnRH analog i.p. at 9 a.m. on day −5. Second injection: 300 μl PMSG i.p. at 1 p.m. on day −3….
Fig. 1 Representative immunofluorescence images of myelinating cultures (28 DIV). (a) Two colour immunofluorescence microscopy reveals the presence of a dense bed of neurites (Red; neurofilament-specific antibody SMI31) and myelinating…
