and Gunhild Waldemar1 (1) Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Abstract The first step in the management of the neurological patient is to localize the lesion. While…
and Gunhild Waldemar1 (1) Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Abstract After finishing the history, the neurologist should have a distinct anatomical hypothesis that can be confirmed…
and Gunhild Waldemar1 (1) Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Abstract Not long ago, neurologists had hardly anything else to offer patients than a diagnosis and words…
and Gunhild Waldemar1 (1) Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Abstract Neurologists take pride in their clinical bedside skills, perhaps even more so than other physicians. Good…
Staining Primary antibody (dilution) Clone Antigen retrieval Blocking solution Secondary antibody (dilution) T cells (mouse) Rat anti-human CD3e (1:50) CD3-12 Citrate pH = 6 10 % FCS + 0.1 % Triton X-100 Biotin anti-rat (1:500) Activated…
Fig. 1 Schematic illustration of the breeding setup used to create a reciprocal backcross population (BC). One pair of autosomes is represented by vertical lines and mitochondria are represented by…
Mouse strain Myelin peptide/protein Sequence Peptide/protein dose (μg) Pertussis Clinical course toxin BALB/c Whole PLP (10) From : bovine 200 Yes Chronic PLP180–199 (10) WTTCQSIAFPSKTSASIGSL 200 Yes C57BL/6 MOG35–55 (11)…
Fig. 1 Bidirectional trans-synaptic axonal degeneration in a hardwired pathway. (a) Normal situation. (b) Axonotmesis of the first axon results in anterograde axonal degeneration (red arrow to the right). This…
Fig. 1 Ventilation system/monitoring setup. A typical setup for intravital microscopy is indicated. Animals are supplied with medical oxygen and isoflurane via a volume-controlled animal ventilator. Physiological parameters are continuously…
MOG Forward 5′-AAA GAATTCGATGGCCTGTTTGTGGAGCT-3′ MOG Reverse 5′-AAA GCGGCCGCCAGGAAGACACAACCATCACTCA-3′ MBP Forward 5′-AAA GAATTCTAGCCTGGATGTGATGGCAT-3′ MBP Reverse 5′-AAA GCGGCCGCCAGGATTCGGGAAGGCTGA-3′ PLP Forward 5′-AAA GAATTCAAGTGCCAAAGACATGGGCT-3′ PLP Reverse 5′-AAA GCGGCCGCGCTCAGAACTTGGTGCCT-3′ All primers were designed with software…
