Staining Primary antibody (dilution) Clone Antigen retrieval Blocking solution Secondary antibody (dilution) T cells (mouse) Rat anti-human CD3e (1:50) CD3-12 Citrate pH = 6 10 % FCS + 0.1 % Triton X-100 Biotin anti-rat (1:500) Activated…
Fig. 1 Schematic illustration of the breeding setup used to create a reciprocal backcross population (BC). One pair of autosomes is represented by vertical lines and mitochondria are represented by…
Mouse strain Myelin peptide/protein Sequence Peptide/protein dose (μg) Pertussis Clinical course toxin BALB/c Whole PLP (10) From : bovine 200 Yes Chronic PLP180–199 (10) WTTCQSIAFPSKTSASIGSL 200 Yes C57BL/6 MOG35–55 (11)…
Fig. 1 Bidirectional trans-synaptic axonal degeneration in a hardwired pathway. (a) Normal situation. (b) Axonotmesis of the first axon results in anterograde axonal degeneration (red arrow to the right). This…
Fig. 1 Ventilation system/monitoring setup. A typical setup for intravital microscopy is indicated. Animals are supplied with medical oxygen and isoflurane via a volume-controlled animal ventilator. Physiological parameters are continuously…
MOG Forward 5′-AAA GAATTCGATGGCCTGTTTGTGGAGCT-3′ MOG Reverse 5′-AAA GCGGCCGCCAGGAAGACACAACCATCACTCA-3′ MBP Forward 5′-AAA GAATTCTAGCCTGGATGTGATGGCAT-3′ MBP Reverse 5′-AAA GCGGCCGCCAGGATTCGGGAAGGCTGA-3′ PLP Forward 5′-AAA GAATTCAAGTGCCAAAGACATGGGCT-3′ PLP Reverse 5′-AAA GCGGCCGCGCTCAGAACTTGGTGCCT-3′ All primers were designed with software…
Fig. 1 After density gradient centrifugation, different blood cells are found in different layers according to their density. From bottom to top you find red blood cells and granulocytes, followed…
Antibody Clone Provider* CD1d LY38 1 CD3 SP34-2 1 CD4 L200 1 CD5 UCHT2 3 CD8 LT8 4 CD9 ML13 1 CD11b D12 1 CD11c S-HCL-3 1 CD16 3G8 1…
Fig. 1 Illustration of full-length MOG fused to EGFP as displayed by the transfected cells. The cartoon of MOG is based on Fig. 1b from (18) Fig. 2 Quantitative analysis…
Fig. 1 Flow cytometric staining of the inflammatory infiltrate at the peak of EAE. (a) CD4 versus CD11b staining of CNS cells (initially gated using a live leukocyte gate). (b)…
