of Transgenic Rats Using Lentiviral Vectors
Fig. 1 Time schedule for oocyte production and retransfer First injection: 200 μl GnRH analog i.p. at 9 a.m. on day −5. Second injection: 300 μl PMSG i.p. at 1 p.m. on day −3….
NEUROLOGY
Cultures: An In Vitro Tool to Identify Demyelinating and Axopathic Autoantibodies
Fig. 1 Representative immunofluorescence images of myelinating cultures (28 DIV). (a) Two colour immunofluorescence microscopy reveals the presence of a dense bed of neurites (Red; neurofilament-specific antibody SMI31) and myelinating…
Light Chain Determination from Peripheral Blood Samples
Fig. 1 Schematic illustration of the electrochemiluminescence NfL immunoassay. The carbon ink electrodes are coated with the capture mAB 47:3. The biotinylated detection mAB 2:1 binds to SULFO-TAG labelled streptavidin,…
Techniques
Fig. 1 Principle of ELISPOT. For cytokine ELISPOT assay, the membrane of an ELISPOT plate is coated first with capture antibody as shown in (a). (b) Cytokines that are produced…
of Central Nervous System (CNS) Infiltrating Cells
Fig. 1 Isolation of CNS infiltrating cells from C57Bl/6 mice with EAE. (a) Absolute numbers of live and CD90 positive cells. Data represents CNS isolated material from 19 EAE diseased…
Critical Care Considerations
Fig. 1 cEEG demonstrating NCSE only detected with depth electrodes (D leads) in a 59-year-old woman with refractory status epilepticus despite pentobarbital, midazolam, levetiracetam, valproate, and phenytoin. Note the relative…
Electrographic Seizures in Adults: Recognition and Examples
Fig. 1 Extreme delta brush pattern (arrow) and seizure onset in a patient with NMDA encephalitis Patients with antibodies to the leucine-rich glioma-inactivated 1 (LGI1) protein component of the voltage-gated…
Super Refractory Status Epilepticus
Stage 1: early SE Seizure lasting 5 min or two or more seizures without recovery of consciousness between Stage 2: established SE Ongoing SE after appropriately dosed benzodiazepine Stage 3:…
QEEG Training Module
Fig. 1 One-hour QEEG panel and corresponding raw EEG. (a) Example of a QEEG panel consisting of the following QEEG tools: rhythmicity spectrogram (displayed for the left and right hemispheres),…
Artifacts Resembling Seizures
Fig. 1 ECG artifact. In this low-voltage EEG, the artifact generated by a brief run of ventricular tachycardia can easily be mistaken for generalized periodic discharges Pulse artifact is another…
