Fig. 1 Pre-surgical MRI to determine surgical coordinates. (a) Animals are anesthetized and fitted into an MRI-compatible stereotaxic head frame by first securing the animal into the ear bars, followed…
Cell number Amount of media for culture Amount of media for a virus infection 1 6 well 1.1 × 106 in one well 2 mL 1 mL 2 T25 3 × 106 4…
Fig. 1 Transduction of neurons and glia in the myenteric plexus of the ENS. Adult male rats received direct injection s of AAV9 (a–d) or AAV6 (e–h) expressing green fluorescent…
Fig. 1 Skull surface sutures and reference points. Stereotaxic surgery utilizes reference points created by the intersection of skull sutures to pinpoint the location of neuroanatomical structures in the brain…
Fig. 1 Diffusion-based delivery results in a limited area of distribution and reflux along the needle track. Increased flow rate with CED results in a pressurized extracellular bulk flow that…
Fig. 1 Time-course analysis of early GL26-Cit glioma growth in the striatum in mice. To visualize perivascular glioma growth GL26-Cit cells were implanted in RA/EGxdelCre mice that express GFP in…
Fig. 1 rAAV2/5 efficiently transduces neurons and produces equal protein expression in the young and aged rat hippocampus (HP). (a–d) Young adult (a and c, 6 months, n = 3) and old…
Model Construct Validity? Reproducible? Progressive nigrostriatal degeneration? Parkinsonian pathology? Parkinsonian symptoms? References Construct validity for idiopathic PD Within group Across groups Striatal DA terminal dysfunction/loss (over weeks) Terminal loss precedes…
Gene knockout Transgenic mice Viral system In vivo electroporation Toxicity No No Potential No Immunogenicity No No Potential No Time specificity No No Yes Yes Site specificity No No…
Fig. 1 Ex vivo delivery of growth factor s targeting skeletal muscles for ALS . A GFP-expressing hMSC line was genetically modified by lentiviral infection to stably release growth factors…
