MRI-Guided Delivery of Viral Vectors
Fig. 1 Infusion system setup: This schematic representation demonstrates all of the components in the infusion system. Starting with a 5 mL syringe (1) mounted onto an infusion pump (2),…
Fig. 1 Infusion system setup: This schematic representation demonstrates all of the components in the infusion system. Starting with a 5 mL syringe (1) mounted onto an infusion pump (2),…
Fig. 1 Photograph of the celiac ganglia under a dissecting microscope. The celiac ganglia (circled) can be located by using the aorta, celiac artery, and superior mesenteric artery as landmarks…
Fig. 1 Setup for free-hand intracerebroventricular injections. (a) Brace arms against the ice bucket for support and stability. Comfortably hold the syringe in one hand with the thumb positioned above…
Fig. 1 An example of a microRNA designed against the human housekeeping gene GAPDH NM_002046.5 (NCBI), both flanks and loop have been modified and adapted from human miR155. (a) Indicates…
Fig. 1 Entry site preference of pseudotyped lentiviral vectors into neuronal cells. Lentiviral vector pseudotyped with VSV-G mainly enters the cell body areas of neurons, whereas the HiRet/NeuRet vectors with…
Fig. 1 Library enrichment for target-specific variants. The DNA encoding the initial capsid library (top left) is cloned into the vector and enters the cycle through which target-specific variants are…
Fig. 1 Intraganglionic injection. Paravertebral surgical exposure for intraganglionic injection, illustrating the operative field (left panel), reference diagram (middle panel), and cleaned vertebral bones (right panel). (a) Initial dissection of…
© Springer Science+Business Media New York 2016Fredric P. Manfredsson (ed.)Gene Therapy for Neurological DisordersMethods in Molecular Biology138210.1007/978-1-4939-3271-9_32 32. AAV2-Neurturin for Parkinson’s Disease: What Lessons Have We Learned? Jeffrey H. Kordower1 (1) Department of Neurological Sciences, Rush…
Fig. 1 Overlapping AAV genome design and transduction. (a) The transgenic cassette, containing the depicted elements, is split into two separate vectors such that there is a homologous sequence overlap…
© Springer Science+Business Media New York 2016Fredric P. Manfredsson (ed.)Gene Therapy for Neurological DisordersMethods in Molecular Biology138210.1007/978-1-4939-3271-9_30 30. Gene Therapy for the Treatment of Neurological Disorders: Metabolic Disorders Dominic J. Gessler1 and Guangping Gao1 (1) University of…